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| Zhang,Fanfan; Ye,Yu; Song,Deping; Guo,Nannan; Peng,Qi; Li,Anqi; Zhou,Xingrong; Chen,Yanjun; Zhang,Min; Huang,Dongyan; Tang,Yuxin. |
| Abstract Background Porcine Deltacoronavirus (PDCoV) is a newly emerged enteropathogenic coronavirus that causes diarrhea and mortality in neonatal piglets. PDCoV has spread to many countries around the world, leading to significant economic losses in the pork industry. Therefore, a rapid and sensitive method for detection of PDCoV in clinical samples is urgently needed. Results In this study, we developed a single-tube one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay specific for nucleocapsid gene to diagnose and monitor PDCoV infections. The detection limit of RT-LAMP assay was 1 × 101 copies of PDCoV, which was approximately 100-fold more sensitive than gel-based one-step reverse transcription polymerase chain... |
| Tipo: Journal article |
Palavras-chave: Porcine Deltacoronavirus (PDCoV); RT-LAMP; Rapid diagnosis. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100222 |
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